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Description

2009;4(3):e4715

TSGIT: an N- and C-terminal tandem tag system for purification of native and intein-mediated ligation-ready proteins

6 were separated from their parents and placed alone in Plexiglas cages for four weeks

This feature is preferable for glutamate production because the accumulated glutamate does not inhibit diazotrophy

Nature 599: 136140.

After sorting, neutrophils were centrifuged at 500 g at 4 C and resuspended in RPMI-1640 medium with 10% FBS for subsequent culture, stimulation and functional assays