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The flowing blood can induce mechanical forces such as shear stress (e.g., laminar or oscillatory flow), acting on endothelial cells and affecting the formation and release of nitric oxide (NO) and ROS and the activation of signal transduction, as well as gene and protein expression that play important roles in vascular homeostasis [24,25]

Negative control cells were incubated in medium only

The old practice of pulling back on the plunger to check for blood is unnecessary for subcutaneous injection

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