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This emphasizes the profound role a diverse subset of microbial-dependent metabolites have on barrier function

In controlled laboratory conditions, it may be included in experimental studies examining peptide stability, structural characteristics, and molecular interaction behavior

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Tight junctions are the fundamental for BBB integrity with very low paracellular leakage, and are critical for controlling hydrophilic molecules [10]

doi:10.1007/BF00805158

10 ul of each drug solution was added to 90 ul of the rat blood serum samples and at specific time intervals the researchers terminated metabolism by adding 1 ml of ACN and acetic acid in order to precipitate the proteins which were then removed after overnight refrigeration followed by centrifugation