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The excessive accumulation of subcutaneous adipose tissue is closely related to metabolic syndrome, cardiovascular diseases, etc

The hAMY 3 R cells were cultured in growth medium consisting of DMEM (Gibco, #31966-021) supplemented with 10% v/v FBS (Gibco, #16140-071), 1% v/v Penicillin-Streptomycin (Gibco, #15140-122), 0.5 mg/mL Geneticin G418 (Gibco, #10131-027), 0.4 mg/mL Hygromycin (Invitrogen, #10687010) and 250 nM Methotrexate (Sigma, A6770)

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10.1016/0002-9343(94)90149-X

FOXO4-DRI is a D-Retro-Inverso (DRI) isoform peptide a synthetic peptide in which the amino acid sequence of the FOXO4 p53-binding domain has been reversed and all L-amino acids substituted with their D-amino acid counterparts