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Tumors were snap-frozen in acetone/dry ice mixture and sectioned (5 m) for blood vessels (anti-CD31 (Abcam, ab28364, 1:50) + AF488 secondary (Abcam, ab150073, 1:400 dilution)) and cellular nuclei (DAPI) staining, and then visualized by confocal microscopy (Zeiss LSM880, v.14.022.021)

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This has been partly due to the unexpected observation of bilateral visual improvement across all gene therapy studies conducted with unilateral IVT injection and the lack of a patient placebo-controlled arm in each of these trials

Month 3+: Maximum benefits usually develop, with continued improvement possible