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The enzyme was loaded to the glutathione-Sepharose column, washed by 20 mM TrisHCl buffer of pH 7.0, and the enzyme was eluted by the same buffer of 20 mM glutathione

YZ: Data curation, Writing review & editing

The osmolytes in the astrocytes provide cellular defense against edema and can be rapidly accumulated or depleted, according to osmotic sensors

PubMed , Scientific Reports , vol

After incubated for the time points described in the figure legends at room temperature, the reactions were arrested by adding the proteoliposome solution to 200 l ice-cold wash buffer and aspirating it through 0.22-m membrane filters (Millipore, MSGVN2210) under vacuum

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