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A deficiency in vitamin B12 can lead to several symptoms, such as fatigue, weakness, anemia, numbness or tingling in the hands and feet, and cognitive difficulties

10.1111/scd.12685 15 de BenoistB

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Following treatment, media was replaced with 80% (v/v) methanol (cooled to 80 C.) containing 500 nmol 6-chloronicotinamide (6-CN) as an internal standard (IS) to extract cellular metabolites

However, like any bioactive compound, understanding the complete safety profile remains crucial for responsible research