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Furthermore, the original co-crystallized ligand inside the active site may affect the conformation of the protein and, as a consequence, the binding mode of other ligands

However, there are limitations to the present study

To experimentally assess the functional consequences of our nrf2a5 mutated allele, we tested its ability to activate the transcription of an ARE-driven firefly luciferase reporter gene following a previously published protocol (Mukaigasa et al, 2012)

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doi: 10.1128/aac.48.11.4414-4421.2004 141 BuchheitKMShawDChuppGLehtimakiLHefflerEFinney-HaywardTet al