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The assay was performed as described by 2.13 Proximity ligation assay (PLA) PC12 cells after fixation were blocked in the Duolink blocking solution in a humidity chamber for 30 min at 37C and incubated with the following primary antibodies: rabbit polyclonal anti-MVI and mouse monoclonal antibodies anti-B23, anti-fibrillarin, anti-UBF, anti-Pol1, anti-p-S6, and anti-S6, diluted in Duolink Antibody diluent solution for 3 h at 37C
& Kraehenbuhl, J
On the other hand, animals defective in occludin have a functional BBB despite the fact that occludin is highly expressed in endothelial cells of the BBB compared to other tissues
Mechanistically, they demonstrated that STUB1 promoted polyubiquitination of GPX4 on lysine 191, leading to its degradation by the ubiquitin proteasome pathway
[125] found that chronic intake of a 60% fat diet in mice led to changes in the dopaminergic pathways