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Hybridoma supernatants were screened 10 days later in a flow cytometry assay for binding to c-His tagged Fsp1 protein captured via biotinylated mouse anti-His antibody (clone HIS 3D5, prepared in-house) to streptavidin beads (PolyAN)

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SPECIAL PRECAUTIONS FOR USE Optimum efficacy can be ensured only when strict hygiene of pig sheds and poultry houses is practiced at treatment

At the nanoscale, the collagen fibrils within the ACL are composed of tropocollagen molecules that assemble into fibrils with specific cross-linking patterns

Thus certain cell types with low levels of GSTs or lacking specific GST isoforms may be at higher risk of AFB 1 toxicity [72]