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Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 g for 5-10 minutes, and the supernatant can be assayed

10.1213/ANE.0b013e318207c51f 204 XieL.JiX.ZhangQ.WeiY

ProcNatlAcadSci USA 2009

Clin Sci 118(9):547564 Peng B-H, Wang T (2019) West Nile virus induced cell death in the central nervous system

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Sergey Terushkin, MD, FACS , a board-certified surgeon with experience in bariatric care, metabolic health, and physician-guided weight management