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doi: 10.1038/s41590-022-01219-w 176 JadhavRRImSJHuBHashimotoMLiPLinJXet al

Subsequently, added 60 L of 50% acetonitrile, vortexed with shaking at 1700 rpm for 10 minutes at 8 C, centrifuged at 15,000 g for 20 minutes at 4 C, and aspirated the supernatant and stored at 80 C until analysis

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