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Activation of ESCRT-III on cell membranes requires Ca 2+ , confirming the role of calcium homeostasis and ERS in the control of ferroptosis (71)

Ramesh A, Archibong AE, Huderson AC, Diggs DL, Myers JN, Hood DB, et al

Co-cultured cells were collected in one 15 ml falcon tube and lysed by RIPA buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS) containing protease inhibitor cocktail (Sigma-Aldrich) and PMSF (Sigma-Aldrich) with 1 M Sodium-Orthovanadate pH 10(Sigma-Aldrich)

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Pereira AAL, Lam M, Kanikarla Marie P, Raghav KPS, Morris VK, Brown H, Windham J, Duose DY, Overman MJ, Vilar Sanchez E, Wistuba II, Kipp P, Janku F, Sinha S, Kopetz S