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The mediators involved in anaphylaxis include: histamine, tryptase, platelet activating factor, prostaglandins, and leukotrienes [20]

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Imidazole looks just like the side chain of histidine residues, so excess concentrations of imidazole, usually 250 to 500 mM imidazole, are used to elute his-tagged proteins from the nickel agarose beads (Figure 3)

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