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Description

De Laere, B

Rylander R, Tallheden T, Vormann J

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10.1038/s41467-022-35337-8 30 BoyleP

VACV secretes approximately 100 immunomodulatory proteins that neutralise cytosolic nucleic acid sensors such as cGAMP synthase, along with several other antiviral response pathways 39

After washing the resin repeatedly with kinase buffer (wash fractions), the protein of interest was eluted using 20 mM glutathione (Bio Basics Canada) and concentrated by centrifugation using 15 kDa Centrifugal Filter Units (Millipore)