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Int Arch Allergy Immunol 175(4):220230 Li W, Deng R, Jing X, Chen J, Yang D, Shen J (2020) Acteoside ameliorates experimental autoimmune encephalomyelitis through inhibiting peroxynitrite-mediated mitophagy activation

They typically involved digestion of genomic DNA via restriction enzymes, cloning and Sanger sequencing

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Each freeze-thaw cycle on reconstituted IGF-1 LR3 reduces potency by 3050% due to ice crystal formation that mechanically denatures the protein structure