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By offering a pragmatic balance between physiological relevance and analytical practicality, millifluidic systems can present a compelling pathway to overcome key bottlenecks in sample yield and workflow integration, thereby accelerating the deployment of these advanced models in drug development and personalized medicine

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In addition to being regulated by HIF1, PDK1 can be activated by two kinases, Akt and PGK1, which are translocated to mitochondria to exert their protein kinase activity ( In addition to energy production, the TCA cycle intermediates, citrate, -ketoglutarate (-KG), succinate, and fumarate, play pivotal roles in tumorigenesis ( Succinate dehydrogenase (SDH) deficiency is another hallmark of metabolic reprogramming in CRC, as a key enzyme linking the TCA cycle and oxidative phosphorylation, SDH activity is critical for maintaining the balance and stability of the electron transport chain ( Thus, the TCA cycle and its associated metabolic alterations have emerged as critical drivers of colorectal cancer pathogenesis, linking mitochondrial function, oncometabolite accumulation, and malignant progression

It also stimulates collagen and elastin synthesis through fibroblast activation, and its copper-chelating properties contribute to antioxidant defense by modulating copper-dependent superoxide dismutase activity

Magnesium chloride and potassium phosphate (dibasic) were from Anachemia (Montral, QC)