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These details matter most for researchers running extended protocols or working with precise dose requirements

This evolution is driven by selective pressure from antibiotic use, with factors such as decades of overuse, incorrect dosing, and the widespread use of antimicrobials in livestock, contributing to the selection of resistant strains

In order to obtain homokaryotic transformants, colonies from single homokaryotic spores were picked and single genomic integration was confirmed by PCR (data not shown) and Southern blot analysis

van der Veen et al., 2017

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