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Many existing studies are limited by small sample sizes or lack of standardized outcome measures

For lipid peroxide evaluation, cells were incubated for another 30 min after being stained with BODIPY 581/591 C11 probe (2.5 M)

To verify the ability of SeC@PA to bidirectionally regulate RS at different GSH levels in vitro, the fluorescent probe 2,7-dichlorofluorescein diacetate (DCF-DA) was used to evaluate RS generation

Glutathione (especially oral forms) can be expensive for long-term use

On the experimental front, the development of stem cell-derived human brain microvascular organoids and BBB-on-a-chip systems offers physiologically relevant in vitro models that recapitulate the cellular complexity and functional properties of the human BBB (244)

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