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Choose less sensitive sites The abdomen is generally the least painful injection site because of its thicker fat layer

Although we still do not fully understand the causes of tumour immune escape and immunosuppressive TME formation, considering the role of fatty acid metabolism in affecting immune capacity, we may be able to focus on combining therapeutic cancer vaccines with drugs targeting fatty acid metabolism of immune cells in the TME to enhance immune activity

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The choice depends on your experimental objectives

Carbonyl formation is routinely exploited to detect the production of reactive oxygen species such as the hydroxyl radical

For instance, studies indicate that mice fed a high-fat diet and treated with MitoQ exhibit minimal metabolic benefits in adipose tissue itself, despite significant improvements in systemic metabolism (Bond et al., 2019)