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Under WD treatment, the marked accumulation of Gly suggests the activation of antioxidant pathways, as Gly is a precursor for glutathione synthesis, a key tripeptide involved in detoxification of reactive oxygen species and maintenance of cellular redox balance (Hasanuzzaman et al

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Caruso I, et al

3K), an increase in liver-body weight ratio (Supplementary Fig

NAPQI rapidly forms adducts with critical cellular proteins, impairs mitochondrial membrane integrity, and results in a profound production of reactive oxygen species (ROS) within hepatocytes 5