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Since most reactions in the second stage are spontaneous, tyrosinase acts as the rate-limiting enzyme in melanin synthesis, making it the primary enzyme involved in both enzymatic browning and melanin production 7

Researchers never published the full results, although a short write-up in 2005 reported that the second trial found a positive effect but that it didnt reach statistical significance

When one is absent, the other provides partial coverage of the reactive estrogen quinone substrate pool