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Based on the skeleton of 2-amino-3-cyano-6-(2-hydroxy-phenyl)pyridine, a series of inhibitors were designed and synthesized, yielding compounds 69-74 [180, 181]

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Briefly, PBS-washed spermatozoa were fixed with Carnoys solution (3:1, methanol and acetic acid) at 4 C for 5 min followed by preparation of smears

In WT mice, the first wave of hepatocyte proliferation is completed within 72 h after two-third hepatectomy