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Ice formation, cryoconcentration, pH shifts, and repeated freeze-thaw cycles can damage peptide integrity or create aggregation

This finding was in accordance with previously reported results (Wang et al., 2020

The carmen Saliare has: "cume tonas Leucesie prai ted tremonti/ quot tibi etinei deis cum tonarem"

Protein J 33(5):403409 de Pinto MC, Locato V, Sgobba A, Romero-Puertas Mdel C, Gadaleta C, Delledonne M, De Gara L (2013) S-nitrosylation of ascorbate peroxidase is part of programmed cell death signaling in tobacco bright yellow-2 cells

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Thats why we check levels first