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Intracellular zinc is required for intestinal cell survival signals triggered by the inflammatory cytokine tnf-alpha

The single chain Fv antibody is a covalently linked V H -V L heterodimer which may be expressed from a nucleic acid including V H - and V L - encoding sequences either joined directly or joined by a peptide-encoding linker

References Lederer DJ, Longo DL, Martinez FJ

Funding This study was supported by a grant (no

While this usually does not pose a significant problem,it may lead to an incorrect estimation of the tagged proteins molecular mass.A further drawback of his-tag-based purification is the co-education of endogenous protein that naturally possess two or more adjacent histidine residues

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