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All animals used in this study were anesthetized with inhalational isoflurane (induction at 4.0% and maintenance at 2.0%) prior to experimental procedures and, when required, were subsequently euthanized by cervical dislocation prior to analysis

doi: 10.1126/science.3029864 60 StockerRGlazerANAmesBN

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What is the relationship between autophagy and necroptosis

Pardoll, D

The ubiquitin ligase RNF8 initiates the ubiquitination of H2AX, and RNF168 amplifies the ubiquitination response, which triggers the recruitment of p53-binding protein 1 (53BP1) and Rap80/BRCA1 to the lesion [92, 93]