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Statistical analysis Statistical analysis was performed using IBM SPSS Statistics 26, and graphs were generated with GraphPad Prism 10

Additional sympathetic and spinal afferent nerves connect the gut to the spinal cord, conveying visceral pain or discomfort and modulating gut immune activity [22]

doi: 10.2183/pjab.84.246

10.1007/s12325-020-01251-y 39 GuoC.LaiL

TRANSCRIPT CLASSIFICATION To assess transcript novelty, the IsoQuant-derived GTF file was analyzed using SQANTI3 v5.5.1( 28 SQANTI3 classified each transcript as known or novel (e.g., NIC, NNC) by comparing it with the reference annotation and reported splice junction quality metrics