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The wells were washed twice with ice-cold PBS, and the cells were lysed by adding 150 l of SDS-PAGE sample buffer followed by sonication and clarification by centrifugation

Large deletions have not been identified, suggesting that complete absence of G6PD might be lethal

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Papanikolaou, Vaia Lambadiari, Journal of Clinical Medicine.2025

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By way of example, two randomized placebo-controlled trials demonstrated that the therapeutic treatment of immune-compromised patients with N-acetyl-cysteine, or NAC, resulted, in both cases, in a substantial growth in most immunological processes which included an entire rejuvenation of natural killer cell activity