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The membrane was washed four times in TBST and visualized using an enhanced chemiluminescence kit (Vazyme, E423-01/02) with a ChemiDoc Touch Gel Imaging System (Bio-Rad)

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In contrast, no significant difference in expression of either gene was observed between nematodes soaked in ds GFP interference solution and those soaked in sterile water ( Figure 6B )

The treatment significantly reduced pro-inflammatory cytokines, including TNF-, TGF-, IL-18, and IL-22, after 6 months compared to untreated controls, suggesting that VE contributes to the anti-inflammatory effects observed ( Contrary to these findings, several studies report a more limited immunomodulatory role for VE in NASH