( D ) N-terminal TDE0362 (N0362, 23204 aa) was constitutively expressed in yeast cells using TEF promoter (p426TEF)
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In accordance with previous results (17), we observe a unique and highly unusual fold for CTL1 among SLC proteins, with 10 transmembrane helices (M1M10), a large extracellular domain bridging M1 with M2, and three shorter amphipathic helices (AH1-3) parallel to the membrane that connect M4M5, M6M7, and M8M9, respectively (Fig 4A)
Several hundreds of early SA-inducible genes were identified including two GSTs
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The solution was purged with nitrogen for 10 min and sealed before being immersed in an oil bath at 65 C for 24 h (Fig