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The record should also clarify that the access was superficial and did not involve deep tissue dissection, as this determines whether 20220 or 20225 applies

The youngest patient I have treated was 15, and the oldest was 82

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Experiments were carried out in PBS (2.7 mM KCl, 137 mM NaCl, 10 mM Na2HPO4 and 2 mM KH2PO4 (pH 7.4)) and 0.001% Tween-20 buffer