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Following DNA end repair and preparation, samples were subjected to a clean-up with MAGBIO beads (MAGBIO Genomics Inc, USA), prior to adapter ligation, as per ONT recommendations

Simultaneously, GLPs effectively modulate blood lipid levels through diverse mechanisms, thereby mitigating fatty liver disease and rectifying the associated dysbiosis of the gut microbiota (Liang et al., 2018

Thus, further research is needed to determine whether strategies that increase SCFA will improve overall treatment outcomes (264)

It is important to highlight that metabolic syndrome and its components are strongly associated with NAFLD/MASLD (20)

These components manage hydration and collagen formation, which are essential for maintaining youthful and resilient skin

To assess the disparities between groups, statistical comparisons were performed employing one-way ANOVA, the KruskalWallis H test and Pearsons 2 test, as appropriate for the specific data type