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10.1016/j.tips.2017.04.005 12 QiY.YaoQ.LiX.LiX.ZhangW.QuP

Sies, Biochem

Defining the SNARE complex binding surface of alpha-SNAP: implications for SNARE complex disassembly

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N-acetyl-p-aminophenol 15

The oligo was dried using a SpeedVac, resuspended in water, applied to a MonoQ 5/50 GL column in buffer A (10 mM TRIS-HCL pH 7.5, 100 mM NaCl) and eluted in a gradient of 3% buffer B (10 mM TRIS-HCL pH 7.5, 800 mM NaCl) per CV at 4 C