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If Phase 2 capacity is insufficient relative to Phase 1 activity, toxin clearance is impaired and symptom burden may increase rather than resolve

The core principle here is creating a solution with a known concentration

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[DOI] [PMC free article] [PubMed] [Google Scholar] 54.TROADEC M-B WARNERD

The -actin protein expression was used as a loading control for each sample after stripping the membrane post-detection with buffer containing 1.5% glycine, 0.1% SDS, and 1% Tween-20 (pH = 2.2)