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The -mercaptoethanol was added freshly and samples were then boiled for 5 min at 95 C

Ubiquitin C-terminal hydrolase-L1 (UCH-L1) shows a similar early increase in blood, and is also detected in cerebrospinal fluid (CSF) throughout 14 days after injury (Svetlov et al., TABLE 3-2 Examples of Biomarkers for TBI 2010)

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Mata-Prez C, Snchez-Calvo B, Padilla MN, Begara-Morales JC, Luque F, Melguizo M, Jimenez-ruiz J, Fierro-Risco J, Penas-Sanjuan A, Valderrama R, Corpas FJ, Barroso JB (2016a) Nitro-fatty acids in plant signaling: nitro-linolenic acid induces the molecular chaperone network in Arabidopsis

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