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Flow cytometry analysis was performed using a FACSCalibur (BD Biosciences)

If youre using a more normal concentration of pH buffer, say 20mM Tris pH 8, then adding 10-20mM glutathione will make your elution buffer more acidic

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doi: 10.1016/j.jff.2024.106476 42

Notably, most of these natural products have undergone extensive study and validation as drugs 56 or food additives 57 , endowing them with heightened clinical translational value owing to their established safety profiles and druggability, in contrast to unexplored chemical structures

These interventions also partially restored gut barrier integrity, reduced renal IgG deposition, and significantly improved kidney function [86]