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8 Streptozotocin (STZ), a glucose analog derived from Streptomyces achromogenes , selectively targets and destroys pancreatic beta cells in rodent models, serving as a widely utilized tool for inducing experimental diabetes

Depressed subjects and stress models reduced BDNF levels, and chronic drug exposure reprogramed its expression 173,174

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Proteins were precipitated again and digested with LysC and trypsin in 200 mM EPPS buffer pH 8.0 at a 100:1 substrate-to-enzyme ratio overnight at 37C