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Xu 2024 (Circulation) Preclinical in vivo, 6 weeks, C57BL/6J mice, pressure-overload TAC heart-failure model

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Non-specific labeling was blocked by incubating scaffolds in 2% BSA solution for 1 h and then samples were immersed in mouse anti-S100 (diluted at 1:1000) overnight

Developed in the 1970s, it remains the most-ordered IV in clinics worldwide

Reported mild side effects include nausea, dizziness, and irritation at the injection site, and the compound is generally well tolerated with minimal side effects in research settings

Although symptoms are included in the definition of PD, it should be recognized that the base definition of PD is fibrosis