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We then tested ATP abundance by liquid chromatographymass spectrometry (LCMS) and show increased ATP levels in cells that survive 5FU treatment (Fig

This protocol uses a 3.0 mL dilution to maintain accurate and consistent dosing per injection

As cancer cells are in a persistent state of oxidative stress with high levels of ROS production, they have a low tolerance thresholds for additional ROS and activation of cell death pathways 17

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S9636), resuspended following the manufacturers instructions, and then subjected to size exclusion chromatography (SEC, Superdex200 16/60) to remove the high level of endotoxin in the material