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ROS-induced mitochondrial dysfunction and DNA damage, along with the inhibition of key redox transcription factors such as Nuclear Factor Erythroid 2-Related Factor 2 (NRF2) may contribute to the persistence of symptoms in both ME/CFS and Long COVID (Jarrott et al., 2022)

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To monitor cell viability, the cells were trypsinized, and apoptosis/necrosis induced by pneumococcal infection was evaluated using flow cytometry with an Annexin V/propidium iodide labeling kit (Invitrogen), showing 5% across all time points

This finding indicates that MND-ART-GEL not only promotes T-cell infiltration but also alleviates T-cell exhaustion and immune suppression, thereby restoring cytotoxic activity

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