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Each group was resuspended by 200 L 1binding buffer containing 2.5 L Annexin V-FITC and 2.5 L 7-AAD (556547, BD Pharmingen, San Diego, USA) for 20 min at room temperature

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PARP cleavage inhibition: PARP, a downstream target of caspase-3, saw its cleavage fragments reduced by 77.5%

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Support Nervous System Function Vitamin B12 plays an important role in maintaining healthy nerves

Collectively, these results suggest that vitamin B 12 becomes systemically depleted during in vivo reprogramming, affecting both the colonic microbiota and the host