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WB performed using the mAb M35 indicated that (1) the recombinant proteins that we obtained are reasonably identical to those secreted by Mtb, since mAb M35 also recognizes and immunoprecipitates the MetE protein present in Mtb culture supernatant, and (2) the epitope recognized by this mAb is located in the more Mtb-specific sequence in the N-terminal fragment of MetE ( Supplementary Figure S4B )
Hinrichs A, Kessler B, Kurome M, Blutke A, Kemter E, Bernau M, Scholz AM, Rathkolb B, Renner S, Bultmann S, Leonhardt H, de Angelis MH, Nagashima H, Hoeflich A, Blum WF, Bidlingmaier M, Wanke R, Dahlhoff M, Wolf E
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