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In the natural aging + FOXO4-DRI group, mice received intraperitoneal injections of FOXO4-DRI (5 mg/kg, MCE, HY-P4157) every 2 days during the same period, followed by tissue collection

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polyclonal rabbit antibodies against Shh, Patched homolog-1 (Ptc-1), smoothened frizzled class receptor (Smo), Gli-1, caspase-3, Bax (1:1000

As we age, collage levels may decline in the body, leading to slower wound healing and increased oxidative stress, all of which lead to aging

doi: 10.1080/13510002.2016.1140406 38 HsuC