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We, therefore, used alternative approaches and have determined the structure of a high molecular weight TrxR subfamily member using cryo-EM, demonstrating the feasibility of this methodology to study this protein subfamily of importance in several human diseases (PDB ID 8A1R, EMD-15084) (Fig

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In animal models of ischemic stroke, VEGF has been shown to improve neuronal survival, reduce infarct size, and enhance cognitive function

All mice were housed under a 12 h light/dark cycle, with the temperature consistently maintained between 18 C and 23 C and the humidity kept at ~50%

The mineral vitamins premix provided the following per kilogram of diet: vitamin A: 6,000 IU, vitamin E: 800 mg, vitamin D3: 4,000 IU, vitamin K3: 30 mg, vitamin B1: 25 mg, vitamin B2: 25 mg, vitamin B6: 80 mg, niacin: 300 mg, vitamin B12: 0.2 mg, pantothenic acid: 200 mg, folic acid: 10 mg, biotin: 4 mg, choline: 5,000 mg

The GSH levels of the BSO-treated cells quantified using our RT-based flow cytometry method is well correlated to the values measured using cell lysates with a glutathione reductase-based biochemical assay ( R 2 =0.99, Fig