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Granulosa cells apoptosis, which disrupts the supportive microenvironment of the oocyte, further accelerates oocyte apoptosis (Zhu et al., 2016

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The cell pellets were cultured in chondrogenic medium (low-glucose Dulbeccos modified Eagle medium containing 0.1 mmol/L ascorbic acid 2-phosphate, 100 nmol dexamethasone, 40 g/mL proline, 100 U/mL penicillin, 100 g/mL streptomycin, and ITS Premix