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Design of the Prostate, Lung, Colorectal and Ovarian (PLCO) cancer screening trial

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For dataset b (GluK2 K676C/N802C supplemented with 1 mM glutamate), the raw movie stacks were motion-corrected using Patch Motion Correction in CryoSPARC4

The barrier index was measured at high temporal resolution of 1 min for 24 h and was normalized to the reference time ( t = 0)

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Spectrometric data were acquired from instruments located at the University of Rhode Island in the RI-INBRE core facility supported by Grant P20GM103430 from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH)