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Because the binding between the epitope and the antibody can occur under the native conditions, any proteins or other macromolecules that are able to interact with the tagged fusion protein will also be captured during the binding step

54 with the following changes: a Bioruptor Plus with automated cooling (4 C) was used for high-intensity sonication (2030 min each with 30 s on and 30 s off intervals) and 10 g MYCN antibody and 10 6 cells for ChIP