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Hyperglycaemia leads to the accumulation of fructose, as mentioned earlier, which is converted to Glucosamine 6-phosphate and ultimately to UDP-N-Acetylhexosamine (UDP-GLCNac), potentiating O-Glucosamine-N-Acetyltransferase (OGT) activity [21]

To ensure that the observed increase in the cells number co-expressing TH and EBF3 was not caused by a general increase in the total number of cells or an increase in the count of cells expressing EBF3, we counted the total number of cells in the SNcVTA region using DAPI-stained nuclei