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In addition, to increase the yield and purity of CihC, the encoding gene was re-cloned into the pET-16b expression vector (Merck, Darmstadt, Germany) by using primers CihC_Nde_FP and CihC_Bam_RP (Table S5)

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To generate mFab/hFc cyclonals with new antigen specificity, V L domain sequences from existing antibodies and antibody fragments 22,41,42,43 were amplified by PCR, while in parallel, mouse C L region DNA was also amplified by PCR

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Someone who has anemia because of their nitrous oxide-induced vitamin B12 deficiency may recover more quickly and fully