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The supernatants from organoids or hepatocytes cultures will be processed the same way for the SomaScan array or Western blotting analysis

PTMs, especially phosphorylation of -syn, affect its iron-binding affinity, which may in turn influence the expression level of -syn protein and the conformation of -syn aggregates, forming a viscous cycle for Iron dysregulation impairs -syn clearance The clearance of intracellular -syn relies mainly on the selective autophagy and proteasome degradation [38]

Proc Am Assoc Cancer Res 45:1304, 2004

doi: 10.1158/1535-7163.MCT-06-0661 61

Kass D, Bridges RS, Borczuk A, Greenberg S

Differential impact of dietary branched chain and aromatic amino acids on chronic kidney disease progression in rats